Comparación de inmunoexpresión de receptores CD68 en células gigantes y mononucleadas en lesiones central y periférica de células gigantes de los maxilares / A comparison of immunoexpression of CD68 receptors in giant cell and mononuclear cells of central and peripheral giant cell lesions of the jaws

Introducción: La lesión central (LCCG) y periférica (LPCG) de células gigantes de los maxilares, son lesiones reactivas con comportamiento clínico diferente. Objetivo: Comparar la inmunoexpresión de CD68 en células gigantes (CGm) mononucleares (CMn) en lesiones central y periférica de los maxilares. Material y métodos: Se evaluaron 35 casos de LCCG y 24 de LPCG en bloques de parafi na que podían ser procesadas para la expresión del anticuerpo CD68. La inmunoexpresión se valoró en el citoplasma de ambas poblaciones celulares, obteniendo proporciones; la inmunoexpresión se categorizó en intensa, moderada, leve. Las proporciones se compararon con χ2, siendo signifi cativo p ≤ 0.05. Resultados: Para las CGm de LCCG, CD68 se expresó en una proporción de 96 versus 84.2% LPCG (p < 0.005). La proporción de la tinción de la expresión intensa y moderada fue más frecuente en las LCCG (p = 0.032). Las proporciones entre las CMn 59.3% LCCG versus 18.6% en la LPCG (p < 0.001). Hubo diferencia en intensidad de CD68, en las CMn de LCCG fue mayor (p = 0.002). Conclusiones: La alta expresión de CD68 en las CGM y CMn en la lesión central y periférica confi rma su fenotipo de macrófago. Las diferencias entre las proporciones y la tinción a CD68 refl eja mayor actividad fagocítica posiblemente relacionada con el comportamiento clínico (AU)

Introduction: Central (CGCL) and Peripheral (PGCL) giant cell lesions of jaws are reactive lesions displaying diff erent behavior patterns. Objective: To compare CD68 immunoexpression between CGCL and PCGL in giant multinucleated and mononuclear cells. Material and methods: 35 CGCL and 24 PGCL were retrieved from paraffi n-embedded biopsy, as well as the feasibility to analyze CD68 immunoexpression. The immunoexpression was analyzed in cytoplasm both cell populations cellular, for and staining intensity was categorized as intense, moderate or faint. Proportions were compared by χ2, making a p ≤ 0.05 value signifi cate. Results: In 96% of CGCL's in GMCs displayed CD68, as compared to 84.2% in PGCL, (p < 0.005). The proportion of stained cells, intense to moderate staining was more frequent in CGCL (p = 0.032). The proportion CD68 was expressed in 59.3% or CGCL mononuclear cells, as compared to 18.6% in PGCL, (p < 0.001). There was diff erence in staining CD68 intensity between mononuclear cells in CGCL, (p = 0.002). Conclusions: The high CD68 expression frequency in GMCs and mononuclear cells in central and peripheral GCL confi rm a macrophage phenotype; a more intense staining in CGML and GMCs suggests a more active phagocytic activity, and possibility underline the diff erent clinical behavior (AU)

Frecuencia y caracterización de Staphylococcus spp. en la mucosa bucal de pacientes diabéticos y no diabéticos / Frequency and characterization of Staphylococcus spp. in the buccal mucosa of diabetic and non-di

Antecedentes: Dentro de la gran diversidad de microorganismos en la microbiota oral, el género Staphylococcus es causante de una gran variedad de infecciones, desde leves hasta diseminadas que pueden causar la muerte. Los portadores de estas bacterias tienen más riesgo de presentar infecciones por estas mismas. Un grupo vulnerable de sufrir infecciones por estos microorganismos son los pacientes diabéticos por sus características sistémicas propias de la enfermedad, deterioro inmunológico y locales bucales, por lo que es importante conocer si son portadoras de este grupo de bacterias. Objetivo: Determinar la frecuencia de colonización por Staphylococcus spp. en aislamientos obtenidos de la mucosa bucal de pacientes diabéticos y sin diabetes. Material y métodos: Se tomó un raspado superficial de la mucosa bucal de personas diabéticas y sin diabetes para cultivo y análisis microbiológico. Se sembró en agar sal manitol y los aislados se identificaron por galerías API Staph. La concentración de glucosa se determinó con equipo Accu-Chek. El análisis fue descriptivo, las diferencias y asociaciones se investigaron con χ2 y T Student. Se consideró estadísticamente significativo cuando el valor de p < 0.05. Resultados: La colonización por Staphylococcus spp. total fue de 73.7%, no hubo diferencia significativa entre diabéticos y no diabéticos (p = 0.946). S. epidermidis se identificó en 69% y S. aureus en 17.6%, sin diferencia entre ambos grupos con p = 0.556 y p = 0.428 respectivamente. Setenta y seis por ciento de los pacientes portadores de prótesis bucales estaban colonizados con Staphylococcus spp. Conclusiones: No se encontró que los pacientes diabéticos tuvieran porcentajes significativamente mayores de colonización por Staphylococcus spp. a pesar de sus condiciones particulares inmunológicas, glucemia anormal y disminución de flujo salival en la cavidad bucal (AU)

Background: Within the great diversity of microorganisms in the buccal microbiota, the genus Staphylococcus is the cause of a great variety of infections ranging from mild to disseminated, which can cause death. The carriers of these bacteria are more at risk of developing infections by themselves. A vulnerable group to suffer infections by these microorganisms are diabetic patients due to their systemic characteristics of the disease, immunological deterioration and local buccal, so it is important to know if they are carriers of this group of bacteria. Objective: The objective was determined the frequency of colonization by Staphylococcus spp. in isolates obtained from the oral mucosa of diabetic and without diabetes patients. Material and methods: A superficial scraping of the buccal mucosa of diabetic and without diabetes people was taken for culture and microbiological analysis. It was seeded in sal manitol agar and the isolates were identified by API Staph galleries. The glucose concentration was determined with Accu-Chek equipment. The analysis was descriptive, differences and associations were investigated with χ2 and Student T. It was considered statistically significant when the value of p < 0.05. Results: Total colonization by Staphylococcus spp. was 73.7%, there was no significant difference between diabetics and nondiabetics (p = 0.946). S. epidermidis was identified in 69% and S. aureus in 17.6%, without difference between both groups with p = 0.556 and p = 0.428 respectively. 76% of patients with oral prostheses were colonized with Staphylococcus spp. Conclusions: Diabetic patients were not found to have significantly higher rates of colonization by Staphylococcus spp. despite their particular immunological conditions, abnormal glycemia and decreased salivary flow in the oral cavity (AU)

Community-acquired methicillin-resistant Staphylococcus aureus can persist in the throat.

Colonization by Staphylococcus aureus is an important factor in infections caused by this microorganism. Among the colonization niches of staphylococci are the nose, skin, intestinal tract, and, recently, the throat has been given relevance. Infections caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) can be fatal. Persistence of S. aureus is an important process in the pathogenesis of this microorganism and must be studied. The aim of this study was to determine the persistence of S. aureus in the throat, and characterized the strains. We studied the persistence of S. aureus for 6 years in the throat of apparently healthy people. The isolated strains from the persistent carriers were characterized through PFGE, spa-typing, SCCmec typing, resistance to methicillin, presence of virulence genes (adhesins and toxins), and the formation of biofilm. We found persistent and intermittent carriers of S. aureus in the throat, with methicillin-sensitive (MSSA), methicillin-resistant (MRSA) strains, and confirmed for the first time that CA-MRSA colonizes this niche. These strains can colonize persistently the throat for four years or more. Typification of strains through PFGE and spa-typing revealed that some carriers present the same strain, whereas others present different strains along the period of persistence. Almost all strains induced a strong biofilm formation. All strains presented adhesin and toxin genes, but no shared genotype was found. We conclude that S. aureus, including CA-MRSA strains, can remain persistently in the throat, finding a wide variability among the persistent strains.

Oral Candida isolates and fluconazole susceptibility patterns in older Mexican women.

OBJECTIVES: To assess the epidemiologic and microbiologic profile and in vitro fluconazole susceptibility of yeasts isolated from the oral mucosa colonization/infection of elderly patients. BACKGROUND: It has been reported that in older adults increases the oral colonization by Candida particularly C. non-albicans, showing a decreased response to fluconazole, which increases the risk of recalcitrant local and disseminated candidiasis. MATERIALS AND METHODS: This was a prospective cross-sectional study conducted in 120 elderly patients. Oral samples were obtained of mucosal Candida colonization or infection by swabbing. Each sample was plated on CHROMagar(®)Candida and incubated (36±1.5°C) for two days. The yeast species were identified using the API(®)ID32-C-AUX. Fluconazole susceptibility was tested using a broth microdilution assay according to the CLSI methods. RESULTS: The yeast colonization/infection frequency in the total population was 65.8%. The frequency of the highest Candida carriers was 67.4% in the 70-79-year-old-group. Oral candidiasis was present in 20%, with a tendency to increase with age (33.3% of adults aged>80years), it was determined that the use of prosthesis is associated with a higher colonization rate (Chi2, p=0.011). The frequency of colonization/infection cases with more than one species showed a tendency to increase with age; 18.9% in the 60-69 year-old-group, 20.9% in the 70-79-year-old-group and 29.2% in the ≥80 year-old-group. About fluconazole susceptibility: for C. albicans, 20.3%, about Candida non-albicans species 15.3% were dose dependently susceptible (DDS) and 17.9% were resistant. CONCLUSIONS: After 80 years of age, there is a considerable increase in Candida non-albicans species and a reduced susceptibility to fluconazole.

Immunohistochemical expression of alpha-smooth muscle actin and glucocorticoid and calcitonin receptors in central giant-cell lesions.

BACKGROUND: Central giant-cell lesions (CGCLs) are reactive lesions that consist histologically of spindle-shaped stromal cells, (fibroblasts and myofibroblasts) loosely arranged in a fibrous stroma, multinucleated giant cells and mononuclear cells with haemorrhagic areas. This study identified the immunoexpression of alpha-smooth muscle actin in spindle-shaped stromal cells, and glucocorticoid and calcitonin receptors in multinucleated giant cells and mononuclear cells. Their association with the clinical and radiographic characteristics of these lesions was identified. METHODS: Thirty-five cases of CGCLs were studied. Expression of alpha-smooth muscle actin, glucocorticoid and calcitonin was evaluated by immunohistochemistry. The labelling index was 100 times the quotient of the number of positive cells divided by the total number of cells of each type. Logistic regression analysis was applied. RESULTS: Alpha-smooth muscle actin was positive (54%) for spindle stromal cells (myofibroblasts). A significant association was observed with root resorption (P = 0.004) and cortical bone destruction (P = 0.024). Glucocorticoid immunoexpression was positive for 99% of the giant cells and 86.7% of the mononuclear cells. Glucocorticoid immunoexpression in the mononuclear cells was associated with root resorption (P = 0.031). A longer evolution time was associated with lower immunoexpression of glucocorticoid (OR 12.4: P = 0.047). Calcitonin immunoexpression was positive in 86% of the giant cells. Immunoexpression of calcitonin was associated with age (P = 0.040). CONCLUSIONS: Myofibroblasts are important components of CGCLs, stromal cells and alpha-smooth muscle. Actin immunoexpression was associated with root and cortical bone resorption.

[Oral lesions associated to immunosuppression in kidney transplant patients]. / Lesiones bucales asociadas a inmunosupresión en pacientes con trasplante renal.

BACKGROUND: The use of immunosuppresive drugs in patients with kidney transplantation (KT) could influence the development of oral lesions (OL). The aim of this study was to establish the OL prevalence in a group of KT patients, and explore some possible associations of their presence. METHODS: Cross-sectional study. We examined the oral mucosa (OM) of 190 KT patients searching for OL. Our findings were analyzed by multiple logistic regression, and possible associations between OLs and several variables (demographic, clinical, of immunosuppressor drugs, and of lab results) were explored. RESULTS: Overall OL prevalence was 28.4 %; 15.8 % had oral candidiasis (which was more prevalent in diabetic cases, p = 0.002), herpes simplex 7.4 %, hairy leukoplakia 5.3 %, oral verruca vulgaris 3.7 %, and OM ulcers 2.6 %. The combination of cyclosporin A + azathioprine + prednisone had the highest OL prevalence. Hairy leukoplakia was related to a lower total leukocyte count, p = 0.006, and oral verruca vulgaris to a cadaveric KT donor. CONCLUSIONS: Oral candidiasis was the most prevalent OL, and it was more prevalent in diabetic cases. The association of hairy leukoplakia to a lower total leukocyte count might agree with previous reports classifying it as an immunosuppression marker.

Introducción: el uso de medicamentos inmunosupresores en pacientes con trasplante renal (TR) predispone el desarrollo lesiones bucales (LB) asociadas a inmunosupresión. El objetivo de este estudio fue determinar la prevalencia de LB en un grupo de pacientes con TR y explorar algunas posibles asociaciones de presencia. Métodos: estudio transversal en el que se examinó la mucosa bucal de 190 pacientes con TR, en búsqueda de LB. Los hallazgos se analizaron mediante regresión logística múltiple y se exploraron posibles asociaciones entre las LB y variables demográficas, clínicas, de los medicamentos inmunosupresores, y de laboratorio. Resultados: la prevalencia de LB fue de 28.4 %; la candidiasis bucal (CB), con 15.8 %, fue más frecuente en diabéticos (p = 0.002); el herpes simple 7.4 %; la leucoplasia vellosa 5.3 %; las verrugas vulgares peribucales 3.7 %, y las úlceras 2.6 %. La combinación de ciclosporina A + azatioprina + prednisona tuvo la mayor frecuencia de LB. La leucoplasia vellosa se asoció a una cifra más baja de leucocitos totales (p = 0.006) y las verrugas peribucales a TR de donador cadavérico. Conclusión: la LB más frecuente fue la CB, la cual fue más frecuente en diabéticos. La asociación de leucoplasia vellosa con una cuenta más baja de leucocitos concuerda con su clasificación previa como marcador de inmunosupresión.

Oral colonisation and infection by Candida sp. in diabetic and non-diabetic patients with chronic kidney disease on dialysis.

INTRODUCTION: Oral candidiasis (OC) is a common opportunistic infection in immunocompromised patients. Species identification is sometimes important for treatment. objective: to determine the prevalence of different Candida species colonising or infecting the oral mucosa (OM) of diabetic (DM) and non-diabetic (non-DM) chronic kidney disease patients, comparing both groups and exploring potential risk factors. METHODS: 56 DM and 80 non-DM patients on chronic dialysis were examined. OM swabs were cultured on Sabouraud dextrose agar plates. Candida species were identified with API® galleries. OC was confirmed by exfoliative cytology. Statistical associations were analysed using χ2, Fisher's exact test (ET), and multiple logistic regression. RESULTS: Candida prevalence was 43.4%: 53.6% DM and 36.3% non-DM, (p=.045). The species identified were C. albicans 74.6%, C. glabrata 22.0%, C. tropicalis 15.2%, C. parapsilosis 3.4 %, C. kefyr 3.4% and C. famata 1.7% without difference between groups. DM patients had a higher xerostomia prevalence (p=.002) and lower salivary flow (p=.008) and lower serum albumin (p=.018). 16.9% of patients had OC, 23.2% DM compared with 12.5% non-DM, (p=.101). The following were associated with the presence of Candida in the OM: the use of dental prostheses (odds ratio [OR] 25.6, 95% confidence interval [CI] 2.5 to 253, P=.001), xerostomia (OR 9.6, 95% CI 2.4 to 38.1, P=.001) and low serum albumin values (OR 0.41, 95% CI 0.22 to 0.98, P=.044). CONCLUSIONS: The presence of Candida sp. in the OM was associated with dental prostheses, xerostomia and low serum albumin.

[Periodontal disease in type 2 diabetes patients with and without chronic renal failure]. / Enfermedad periodontal en pacientes diabéticos con y sin insuficiencia renal crónica.

OBJECTIVE: To estimate and compare periodontal disease prevalence (PD) in two groups of patients with type 2 diabetes (T2D), with and without end stage renal disease (ESRD) and dialysis. MATERIAL AND METHODS: Using a cross sectional design, two groups of patients with type 2 diabetes were examined in two general hospitals belonging to Instituto Mexicano del Seguro Social in San Luis Potosí. Group A were patients with ESRD and dialysis; Group B, patients with serum creatinine < 2.0 mg/dL. The WHO Community Periodontal Index (CPI) was used to assess PD. 233 patients were examined among which 172 had functional tooth, suitable for PD evaluation. RESULTS: 71 patients integrated group A; their mean age was 56.2 +/- 11.8 years. 101 patients integrated group B, their mean age was 56.7 +/- 11.9 years (p = 0.768). Elapsed time from T2D diagnosis was 221 +/- 86 months for group A, and 126 +/- 101 months for group B (p < 0.001). Poor oral hygiene was found in 39.8 % of group A patients, and 34.6 % of group B patients (p = 0.133). PD prevalence, with CPI scores 3 and 4, was 63.4 % for group A, and 72.3 % for group B (p = 0.216). Multiple linear regression analysis found PD severity associated to ageing (p < 0.001), poor oral hygiene (p < 0.001) and for group A a shorter time from T2D diagnosis to ESRD (p = 0.0315). ESRD hemodialysis patient's PD was more severe than that of peritoneal dialysis patients (p < 0.03). CONCLUSIONS: both groups had high prevalence of PD and poor oral hygiene. Patients treated with haemodialysis had more severe PD than peritoneal dialysis patients. A shorter time from DM diagnosis to ESRD was found associated to more severe PD.

Oral mucosa symptoms, signs and lesions, in end stage renal disease and non-end stage renal disease diabetic patients.

AIM: To assess oral signs, symptoms and oral lesions (OL) type and prevalence, in diabetic patients with end stage renal disease (ESRD DM), and compare them with analogous findings in a non-ESRD DM group; analyze the possible association between oral manifestations, as well as with relevant laboratory findings. Research design. Two adult groups were studied: Group A: ESRD DM on dialysis, and group B: non-ESRD DM (serum creatinine <2.0 mg/dl). Known DM evolution time, dialysis treatment type and duration, and laboratory results were recorded. An oral exam was performed, searching for signs, symptoms and ESRD-associated OL. Associations were analyzed using Chi square, Fisher s exact test, and odds ratios (OR) with 95% confidence intervals. Ages, time on dialysis, and laboratory results were compared with Student s t test. RESULTS: 229 individuals were examined, group A 99, and group B 130 pts. Signs and symptoms prevalence was higher in group A: 77.8% vs. 57.6%, (P<0.001), uremic breath (48.5%), unpleasant taste (45.5%) and xerostomia (44.4%) being the most frequent ones. OL were also more prevalent in group A; 65.6% vs. 36.9% (P<0.001). The most frequent OL were dry, fissured lips (28.3%), saburral tongue (18.2%) and candidiasis (17.2%). No difference was found in candidiasis prevalence between groups. Candidiasis was found associated to xerostomia (P<0.05) and smooth tongue (P<0.05) only in group A. CONCLUSIONS: ESRD DM patients had a significantly higher prevalence of signs, symptoms and OLs, as compared to non-ESRD DM pts. The high prevalence of uremic fetor, xerostomia, saburral tongue and candidiasis in group A, could be tried as warning signs on the possibility of non diagnosed advanced renal disease in other diabetic patients.

Graft-versus-host disease, an eight case report and literature review.

UNLABELLED: Graft versus host disease (GVHD) is a common complication in bone marrow transplant (BMT) patients. It is characterized by systemic and oral cavity alterations. Depending on the timing of lesions, GVHD is classified as acute or chronic. Alterations in the oral cavity are lichenoid reticular lesions, erythema, ulcerations, and xerostomia. Sporadically, mucocele and pyogenic granulomas can be present. AIM: To describe GVHD oral manifestations in eight allogenic BMT patients, and discuss GVHD and drug-immunosuppresion associated lesions diagnosis and treatment. STUDY DESIGN: For a year, we examined the oral mucosa of eight consecutive allogenic BMT patients attending the Dermatology out-patient clinic at the Instituto Nacional de Cancerologia (National Institute of Oncology) in Mexico City, looking for oral mucosa lesions. RESULTS: Patients were five men and three women, ages 24.8 -/+ 9.7 years. Four had a BMT because of chronic granulocytic, two for acute myeloblastic, one for acute lymphoblastic leukemia, and one for aplastic anemia. Three patients developed acute GVHD, with reticular oral mucosa lesions, erythema and mucositis; and all eight developed chronic GVHD, with reticular oral lesions, erythema, and ulcerations. A Patient had tongue and cheek pyogenic granulomas. Six reported xerostomia. Other oral lesions, associated to drug-immunosuppression, were candidiasis and herpes simplex. CONCLUSIONS: Patients with GVHD frequently develop oral lesions, some of which interfere with normal feeding; timely diagnosis and treatment are therefore essential to improve the quality of life of affected patients. We propose an alternative treatment for pyogenic granulomas.

Oral lesions in a group of kidney transplant patients.

AIM: To assess the prevalence of oral mucosa lesions (OL) in a group of kidney transplant (KT) patients, and analyze possible OL associations with one another and with drugs use and relevant clinical and laboratory variables. METHODOLOGY: Transversal study, in which consecutive KT patients from the nephrology outpatient service at a General Zonal Hospital were examined. The prevalence of several types of OL was assessed, and their possible statistical associations with one another and total leukocyte count, renal function, the simplified oral hygiene index (S-OHI), cyclosporin-A (CsA) dose and blood levels, and nifedipine use was analyzed. A logistic regression model was used to analyze the association between gingival hyperplasia (GH) and CsA dose and nifedipine use. RESULTS: Fifty (55.6%) men and forty (44.4%) women were studied. Sixty percent of the patients had at least one OL. Oral candidiasis (OC) was found in 18.7%; 13% had lesions clinically compatible with hairy leukoplakia (CHL). An association was found between OC and CHL (P<0.05). Saburral tongue (ST) was found in 22% of the patients and gingival hyperplasia (GH) in 49%, which was distributed as follows: Grade 1 in 11 (12.2%); Grade 2 in 26 (28.9%), and Grade 3 in 7 (7.8%). Logistic regression results showed an association between GH and poor oral hygiene (P< 0.001), but not to either CsA dose or blood levels, or nifedipine use (P=0.075). CONCLUSION: Sixty percent of the KT patients had at least one OL. The association between GH and poor oral hygiene corroborate the need for oral hygiene practices supervision in the transplant patient.

Manifestaciones bucales en pacientes VIH-positivos en la ciudad de México: informe preliminar / Oral manifestations in HIV-positive patients in Mexico city: a preliminary report

Informe preliminar que describe las manifestaciones bucales encontradas en 54 pacientes VIH-positivos examinados en la clínica de SIDA del INNSZ, de los cuales 46 (85 por ciento) se encontraban en estadio avanzado de la infección (CDC-IV) y 8 (15 por ciento) en estadios tempranos (CDC-II y III). El 81 por ciento de los individuos examinados presentaron una o varias lesiones bucales. La candidosis bucal (46 por ciento) y la leucoplasia vellosa (35 por ciento) fueron las más frecuentes. La candidosis eritematosa se observó con mayor frecuencia (31 por ciento) que el tipo paseudomembranosa (15 por ciento): asimismo la primera se observó asociada a estadios tempranos y la segunda a etapas tardías de la infección. La frecuencia de leucoplasia vellosa se observó aumentada en el grupo CDC-IV. Si bien el presente trabajo es tan sólo un informe preliminar, cabe afirmar que las manifestaciones bucales son frecuentes en pacientes en etapas tempranas y tardías de la infección por VIH